Liu (2020)
Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020
https://doi.org/10.1016/j.cca.2020.03.009
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094385/
Patient characteristics
Age (1 year increase vs. Not applicable)
SARS COV-2 infection
Odds ratio: 1.033 (1.029-1.037) Adjusted model

China

Retrospective observational study

Medical records

4880

Analysed viral nucleic acid test (NAT) of 4880 cases from Jan 22 to Feb 14, 2020 were tested for SARS-CoV-2 infection in Renmin Hospital of Wuhan University.

The male patients are 2251, female are 2629. There is no obvious gender difference during sample collection. But for male, 40.43% were positive while for female, 36.71% were positive. All the cases were suspected of SARS-CoV-2 infection because of, 1) typical respiratory infection symptoms such as fever, cough and dyspnoea, or 2) close contact with a COVID-19 patient. RT-PCR were performed to detect ORF1ab and NP genes fragments from respiratory specimens, including nasal and pharyngeal swabs (NPS), bronchoalveolar lavage fluid (BLF) and sputum.

0


SARS COV-2 infection

1875

The presence of SARS-CoV-2 in respiratory specimens was detected by real-time RT-PCR amplification of SARS-CoV-2 open reading frame 1ab (ORF1ab), nucleocapsid protein (NP) genes fragments using kits provided by Shanghai Huirui Biotechnology Co.,Ltd. Conditions for amplifications were 50°C for 15 min, 95°C for 3 min, followed by 45 cycles of 95°C for 15 s and 60°C for 30 s


Patient characteristics

Age

Positive rate increased from 24.90% (age 18-30) to 61.81% (age >70).

Not applicable

1 year increase


Odds ratio

1.033 (1.029-1.037)

No

Yes

Yes

Adjsuted for age and gender


none

Good

Yes