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Article data
Liu (2020)
Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020
https://doi.org/10.1016/j.cca.2020.03.009
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7094385/

Results
Patient characteristics
Age (1 year increase vs. Not applicable)
SARS COV-2 infection
Odds ratio: 1.033 (1.029-1.037) Adjusted model

Study characteristics

China

Retrospective observational study

Medical records

4880

Analysed viral nucleic acid test (NAT) of 4880 cases from Jan 22 to Feb 14, 2020 were tested for SARS-CoV-2 infection in Renmin Hospital of Wuhan University.

The male patients are 2251, female are 2629. There is no obvious gender difference during sample collection. But for male, 40.43% were positive while for female, 36.71% were positive. All the cases were suspected of SARS-CoV-2 infection because of, 1) typical respiratory infection symptoms such as fever, cough and dyspnoea, or 2) close contact with a COVID-19 patient. RT-PCR were performed to detect ORF1ab and NP genes fragments from respiratory specimens, including nasal and pharyngeal swabs (NPS), bronchoalveolar lavage fluid (BLF) and sputum.

0


Results

SARS COV-2 infection

1875

The presence of SARS-CoV-2 in respiratory specimens was detected by real-time RT-PCR amplification of SARS-CoV-2 open reading frame 1ab (ORF1ab), nucleocapsid protein (NP) genes fragments using kits provided by Shanghai Huirui Biotechnology Co.,Ltd. Conditions for amplifications were 50°C for 15 min, 95°C for 3 min, followed by 45 cycles of 95°C for 15 s and 60°C for 30 s

Patient characteristics

Age

Positive rate increased from 24.90% (age 18-30) to 61.81% (age >70).

Not applicable

1 year increase

Odds ratio

1.033 (1.029-1.037)

No

Yes

Yes

Adjsuted for age and gender


Other information

none

Good

Yes

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