Liu (2020)
Positive rate of RT-PCR detection of SARS-CoV-2 infection in 4880 cases from one hospital in Wuhan, China, from Jan to Feb 2020
Patient characteristics
Age (1 year increase vs. Not applicable)
SARS COV-2 infection
Odds ratio: 1.033 (1.029-1.037) Adjusted model


Retrospective observational study

Medical records


Analysed viral nucleic acid test (NAT) of 4880 cases from Jan 22 to Feb 14, 2020 were tested for SARS-CoV-2 infection in Renmin Hospital of Wuhan University.

The male patients are 2251, female are 2629. There is no obvious gender difference during sample collection. But for male, 40.43% were positive while for female, 36.71% were positive. All the cases were suspected of SARS-CoV-2 infection because of, 1) typical respiratory infection symptoms such as fever, cough and dyspnoea, or 2) close contact with a COVID-19 patient. RT-PCR were performed to detect ORF1ab and NP genes fragments from respiratory specimens, including nasal and pharyngeal swabs (NPS), bronchoalveolar lavage fluid (BLF) and sputum.


SARS COV-2 infection


The presence of SARS-CoV-2 in respiratory specimens was detected by real-time RT-PCR amplification of SARS-CoV-2 open reading frame 1ab (ORF1ab), nucleocapsid protein (NP) genes fragments using kits provided by Shanghai Huirui Biotechnology Co.,Ltd. Conditions for amplifications were 50°C for 15 min, 95°C for 3 min, followed by 45 cycles of 95°C for 15 s and 60°C for 30 s

Patient characteristics


Positive rate increased from 24.90% (age 18-30) to 61.81% (age >70).

Not applicable

1 year increase

Odds ratio

1.033 (1.029-1.037)




Adjsuted for age and gender